Tuesday, September 17, 2019
Characterization and Quantification of Active Compounds of Black pepper (Piper Nigrum): AbstractThe objectives of the study were to investigate the presence of phytochemical compounds from the extract of piper nigrum, using methanolic extraction. It showed the presence of alkaloid, flavanoids, carbohydrates, proteins, phenols, phytosterols, and tannins. The phytochemical compounds were also screened by GC-MS method.A total 21 bioactive compounds were identified in the methanolic extract of Piper nigrum. The identification of phytochemical compounds is based on the peak area,retention time,molecular weight.GC-MS analysis of P.nigrum revealed the existence of major compounds like caryophyllene ,delta.3-carene as well as Cinene. The results will be useful for further study in future to explore the biosynthetic activity of black pepper. Introduction:Black Pepper:Black Pepper, the tropical plant family, Piperaceae is a reliable source of diverse biologically dynamic phytochemicals (Parmar et al., 1997) and food grade spice. Black pepper (Piper nigrum L.) (Piperaceae) celebrated as Ã¢â¬Å"Dark GoldÃ¢â¬ and furthermore known as Ã¢â¬Å"Ruler of SpicesÃ¢â¬ is one of the vital rural products (Ankegowda and Krishnamurthy 2012). The extracts of Pepper can be viably used as a source of biopesticide material. The most broadly perceived specie of this family is Piper Nigrum or dark pepper which apart from culinary applications is utilized as a part of various ayurvedic details because of its different curative properties (Srinivas et al., 2005). Piperine is one of the significant compounds that is extracted from Piper Nigrum. Many other unsaturated amides have since been distinguished in Piper species from every single tropical locale of the world (Parmar et al 1997). Piperine is the significant compound that causes the impactful and spicy flavor of pepper and has indicated many pharmacological exercises, for example, insecticidal, calming, pain relieving, and so forth. (Khajuria et al., 1989). The wide variety of auxiliary plant mixes found in Piper were recommended as potential leads for novel bug sprays (Miyakado et al., 1989) while numerous mixtures are utilized as a part of conventional control of insecticides that are vectors of ailment (Okorie and Ogunro 1992) and harm stored crops (Sighamony et al. 1986; Baier and Webster 1992; Mbata et al., 1995; Ke'?Ã ¨ta et al., 2000). Early examinations with P. nigrum seed extracts showed that piperamides were in charge of the harmfulness of the extracts to the adzuki bean weevil Callosobruchuschinensis L. (Miyakado et al., 1980). For the treatment of infectious diseses on Earth pharmacologists ,microbiologists and natural products chemists are relying on plant derived dietary supplements as well as phytochemicals.(Pandey et al.,2015).The objective of this research is to identify and characterize the active compounds in crude extract of Black pepper. Key words: Piper nigrum L, Phytochemical Screening,GC-MS.Material and Method:The seeds of Black pepper were purchased from Qissa Khwani Bazar PeshawarExtract of Black Pepper: Seeds of black pepper 10gm were grounded in a blender and then covered with 100 ml of methanol. The grounds and methanol slurry was shaker in a mini orbital shaker for 30 min at 260 rppm and then passed through a Buchner funnel with Whatman No.1 filter paper for removing insoluble material. The methanol fraction was separated and air died ( Jyothiprabha, and Venkatachalam 2016). Identification of Active Compounds: Some plants have more than one chemical as an active principle responsible for their biological properties. Phytochemical tests was done to find the presence of the active chemical constituents such as alkaloid, glycosides, flavonoids, reducing sugars, triterpenes, phenolic acid and tannis. Detailed Phytochemical screening was carried out as per standard methods. (Kokate, 2000; Habone, 1999; Tiwari et al., 2011).